三重四极杆质谱仪上安装反吹压力控制三通

Abstract: 7000系列三重串联四极杆质谱仪设计的目的是为气相色谱提供最高的灵敏度和选择性。气相色谱/质谱/质谱选择性的增强简化了样品前处理过程，从而提高了样品通量并降低了样品周转时间。然而，这些更复杂的样品会迅速降低气相色谱(GC)和质谱仪(MS)的性能。应用反吹技术可以在下一个样品进样之前，直接除去柱中的后洗脱化合物以防止其进入质谱仪，从而帮助我们保持气相色谱和离子源性能。本文介绍了应用配置有压力控制三通反吹技术的 Agilent 7890 GC 串联 7000系列三重串联四极杆质谱，进行选择反应监控实验的几个例子。结果表明，在八氟萘的 272→241 m/z检测中，峰面积、峰高度或者信噪比中没有灵敏度和精确度损失。这就意味着，利用一个简单的压力控制三通配置，在没有排空质谱系统的情况下，快速反吹和快速柱、进样口维护可以合并进行而不带来检测限的损失。 The 7000 Series Triple Quadrupole Mass Spectrometer is designed to provide the most sensitive and specific analytical determinations in gas chromatography. The enhanced specificity of GC/MS/MS analysis encourages a more relaxed or minimized sample preparation strategy with the aim of higher sample throughput and sample turnaround time. However these more complex samples can rapidly degrade the gas chromatograph (GC) and mass spectrometer (MS) performance. Applying backflushing helps maintain the GC and the MS source by removing later eluting compounds from the column before reaching the MS, and prior to the next sample injection. This note cites the results of the Pressure Controlled Tee (PCT) configuration for rapid and universal GC/MS backflushing applied to selected reaction monitoring experiments on the Agilent 7890 GC used with the Agilent 7000 Series Triple Quadrupole Mass Spectrometer. The results show no loss in sensitivity and precision as measured for the peak area, height or signal-to-noise ratio in the 272&241 m/z for octafluoronaphthalene. This means that rapid backflushing and quick column and inlet maintenance without venting the MS system can be incorporated without a loss in detection limits using a simple PCT configuration.

Keywords: 反吹，三重四极杆，灵敏度，多反应监测，7000 Backflush, Triple Quadrupole, Sensitivity, MRM, 7000
Publication Number: 5990-4504CHCN
Last Updated: 9/3/2010
Number of Pages: 4

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来源：实验与分析