Agilent 2100 生物分析仪检测转基因生物

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Detecting genetically modified organisms with the Agilent 2100 bioanalyzer

Abstract: Labeling of food containing more than 1% of genetically modified organisms (GMOs) has been obligatory in Europe since January 2000. To guarantee transparency and labeling, methods to distinguish between transgenic food and their traditional counterparts must be available. Genolife developed a method to detect Ready RoundUp soy (RRS) and a multiplex PCR to detect five corn transgenes (Bt176, Bt11, MON810, T25 and GA21). The Agilent 2100 bioanalyzer and DNA 500 LabChip® kit provided a simple, high throughput and standardized way to analyze multiplex PCR products. The methods developed by Genolife allow detecting 0.01% of RRS in food ingredients on the one hand and 10 copies of transgene MON810, GA21 and Bt11, and 100 copies of transgene Bt176 and T25 on the other.

Keywords: 2100 bioanalyzer, genetically modified organisms, GMO, DNA 500 LabChip kit
Publication Number: 5988-4847EN
Last Updated: 4/9/2010
Number of Pages: 4

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来源：实验与分析