Bravo自动化液体处理平台联合应用

Abstract: 安捷伦科技的自动化解决方案部门和Stratagene 部门联合开发了利用Bravo 自动化液体处理平台和Absolutely RNA 96 孔板微量提取试剂盒以实现在96 孔板中自动化抽提RNA 的新方法。现在RNA 抽提方法需要大量人工操作，包括重复性的移液和真空抽滤步骤。使用安捷伦VWorks 自动化控制软件，可以编制一个程序完成吸液，分液，混匀，从96 孔组织培养板转移样品至96 孔全基因组RNA 试剂盒吸附收集板，还能应用Bravo 平台上配备的真空抽滤模块来自动完成样品洗脱的步骤。最后，可以利用安捷伦的2100 生物分析系统对RNA 的质量进行评估。 A new method was developed to automate RNA isolation in a 96-well format using the Bravo Automated Liquid Handling Platform and Absolutely RNA 96 Microprep Kit from Agilent Technologies (Agilent Automation Solutions and Stratagene divisions, respectively). Current RNA isolation protocols require extensive manual processing including repetitive pipetting and vacuum filtration steps. Using Agilent’s VWorks Automation Control Software, a script was written for the Bravo Platform to aspirate, dispense, mix, and transfer sample from a source 96-well tissue culture plate to the binding and collection plates of the Absolutely RNA 96 kit; as well as to automate sample wash steps using an accessory vacuum filtration unit on the Bravo Platform. RNA quality was assessed on the Agilent BioAnalyzer 2100.

Keywords: VWork,生物分析仪，2100，离心机，Stratagene Absolutely RNA 96 孔板微量提取试剂盒，RNA分离，自动化，Bravo VWork, BioAnalyzer, 2100, Microplate, Centrifuge, Stratagene Absolutely RNA Microplate Prep, RNA isolation, automation, Bravo
Publication Number: 5990-3558CHCN
Last Updated: 12/28/2009
Number of Pages: 4

View Article (0.65 MB)

For your convenience this PDF file will open in a separate window, and you may need to adjust your pop up blocker setting.

来源：实验与分析